Shock Wave Therapy Promotes Cardiomyocyte Autophagy and Survival during Hypoxia

Shock Wave Therapy Promotes Cardiomyocyte Autophagy and Survival during Hypoxia

Blog Article

Background: Autophagy plays an important role in cardiovascular disease.Controversy still exists regarding the effect of autophagy on ischemic/hypoxic myocardium.Cardiac shock wave therapy (CSWT) is an effective alternative treatment for refractory ischemic heart disease.

Whether CSWT can regulate cardiomyocyte autophagy under hypoxic conditions is not clear.We established a myocardial hypoxia model using the H9c2 cell line and performed shock waves (SWs) treatment to evaluate the effect of SW on autophagy.Methods: The H9c2 cells were incubated under hypoxic conditions, and SW treatment was then performed at energies of 0.

02, 0.05, or 0.10 mJ/mm2.

The Toy Trains cell viability and intracellular ATP level were examined.Western blot analysis was used to assess the expression of LC3B, AMPK, mTOR, Dish Caddies Beclin-1, Sirt1, and HIF-1α.Autophagic vacuoles were visualized by monodansylcadaverine staining.

Results: After the 24-hour hypoxic period, cardiomyocyte viability and ATP levels were decreased and autophagy was significantly increased in H9c2 cells.SW treatment with an energy of 0.05 mJ/mm2 significantly increased the cellular viability, ATP level, LC3B-II/I, and number of autophagic vacuoles.

In addition, phosphorylated AMPK and Sirt1 were increased and phosphorylated mTOR and HIF-1α were decreased after SW treatment.Conclusion: SW treatment can potentially promote cardiomyocyte autophagy during hypoxia and protect cardiomyocyte function by regulating the AMPK/mTOR pathway.